ZR Genomic DNA™ -Tissue MiniPrep

ZR Genomic DNA™ -Tissue MiniPrep

For high quality DNA purification from solid tissues (e.g., tailsnips, earpunches, adipose tissue, etc.), whole blood, plasma, serum, buffy coat, lymphocytes, cultured cells, buccal cells, FFPE tissues, semen, hair, and other biological sources
Combines Proteinase K digestion with innovative Fast-Spin column purification technology.
Isolated DNA is ideal for PCR, endonuclease digestion, Southern blotting, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
Product Name Size Catalog #         How to Buy
ZR Genomic DNA™ -Tissue MiniPrep            50 Preps.         D3050 info@nanodiagnostika.lt
ZR Genomic DNA™ -Tissue MiniPrep 200 Preps. D3051 info@nanodiagnostika.lt

 

Specifications

Format Spin Column
Processing Time 25 min
Sample Sources Solid tissues (e.g., tailsnips, earpunches, adipose tissue, etc.), whole blood, plasma, serum, buffy coat, lymphocytes, cultured cells, buccal cells, FFPE tissues, semen, hair, and other biological sources are effectively processed using this kit.
DNA Purity High quality DNA for PCR, endonuclease digestion, Southern blotting, bisulfite conversion/methylation detection, sequencing, genotyping, etc., is eluted with DNA Elution Buffer or water (A260/A280 ≥1.8).
Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS
DNA Yield The DNA binding capacity of the column is 25 µg. Typically, mammalian tissues yield: 1 - 3 µg DNA per mg skeletal, heart, and brain tissues and 3 - 5 µg DNA per mg liver, kidney and lung tissues. Human whole blood will yield 3 - 7 µg DNA per 100 µl blood sampled. DNA is eluted into ≥ 50 µl DNA Elution Buffer or water.
Equipment Needed Water bath or heat block (55°C), microcentrifuge, and vortex.
DNA Size Capable of recovering genomic and mitochondrial DNA sized fragments from 100 bp to ≥ 40 kb. If present, Parasitic, microbial, and viral DNA will also be recovered. Typical fragment sizes range from 25 kb - 35 kb.

Manufacturer: Zymo Research

 
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