Afyon SDS-PAGE mėginio paruošimo rinkinys

Afyon SDS-PAGE sample preparation kit

  • pašalina chemikalus, kurie gali trukdyti elektorforezei (GuHCl, urea, amonio sulfatas ir kt.)
  • baltymų mėginiai paruošiami per mažiau nei 10 min
  • saugus ir netoksiškas (be DMSO)
  • daug greitesnis metodas nei koncentravimas ultrafiltravimo ar dializės būdu
  • Suderinamas su SDS-PAGE ir Western blotting tyrimais

Kat. Nr. Produkto pavadinimas Pakuotės dydis      Kur įsigyti
R-03018-B10 Non-reducing protein sample loading buffer (2X) 1 ml info@nanodiagnostika.lt
K-02101-010 Afyon SDS-PAGE Sample Preparation Kit 10 rxns
K-02101-025 Afyon SDS-PAGE Sample Preparation kit 25 rxns-/-
  • Description
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The Afyon SDS-PAGE sample preparation kit provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis. The fast, efficient protocol generates samples ready to load on a gel in less than ten minutes, much more quickly than can be achieved with alternate methods such as dialysis, or acetone or TCA precipitation. Additionally, the Afyon protocol is easily scaled up, allowing multiple samples to be prepared in parallel.

Afyon Figure 1

 

Afyon concentration is compatible with Western blot detection. Western blots were created with samples of A431 cell extract (panels a and b, lane 2), diluted cell extract (lane 3) and the diluted extract concentrated using the Afyon protocol (lane 4). The blots were detected using the WesternBright MCF multicolor fluorescent Western blotting kit. No bands were detectable in the diluted cell extract when stained for either GAPDH (panel a, lane 3) or SRC (panel b, lane 3). However, after Afyon concentration, bands are easily visualized for both proteins (lanes 4). Band intensities are indicated.

 
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